DOI:10.3969/j.issn.1672-1993.2021.04.0444

·皮肤性病学·

miR-203 和 p63 在尖锐湿疣男性患者组织中的表达及意义

高云¹ 柯锦²△

1 鄂州市疾病预防控制中心皮防所，湖北 鄂州 436000

2 上海市浦东医院皮肤科，上海 201399

【摘要】目的 检测微小RNA-203(miR-203)及其下游靶基因p63在尖锐湿疣(CA)组织中的表达水平，探讨其在CA治疗中的意义。方法 选取2017年9月至2018年12月鄂州市疾病预防控制中心皮防所诊治的40例CA男性患者作为研究对象。将其纳入CA组。选取同期行包皮环切术的40例男性患者纳入对照组。采用实时荧光定量PCR法检测受试者miR-203和p63的表达，HE染色观察组织形态学特征，免疫组化法检测CA组织中p63蛋白的表达。结果 HE染色观察CA组织表皮出现乳头瘤样增生，颗粒层出现空泡化细胞均为CA的典型组织形态学特征。与对照组比较，CA组miR-203表达水平下降，p63 mRNA表达水平升高，p63蛋白阳性率升高(P＜0.05)。CA组p63蛋白表达与临床病理指标关系进行分析，p63蛋白表达水平与患者年龄、发病部位和HPV DNA载量无相关性(P＞0.05)，与HPV感染类型有关(P＜0.05)。CA组miR-203表达与p63蛋白表达呈负相关(P＜0.05)。结论 miR-203在CA组织中表达显著降低，p63表达显著升高，miR-203与p63表达呈负相关，可能影响CA发生。

【关键词】尖锐湿疣；微小RNA-203；p63；临床病理

【中图分类号】R759

【文献标识码】A

Expression and significance of miR-203 and p63 in tissues of male patients with condyloma acuminatum

GAO Yun¹, KE Jin²△. 1. Institute of Dermatology, Ezhou Center for Disease Control and Prevention, Ezhou 436000, Hubei,China; 2. Department of Dermatology, Shanghai Pudong Hospital, Shanghai 201399,China

【Abstract】Objective To detect the expression of microRNA-203 (miR-203) and its downstream target gene p63 in the tissues of condyloma acuminatum (CA), and to explore its significance in the treatment of CA. Methods 40 male patients with CA diagnosed and treated in the Institute of Dermatology, Ezhou Center for Disease Control and Prevention from September 2017 to December 2018 were included in the CA group, and 40 male patients who underwent circumcision in the same period were included in the control group. The expression of miR-203 and p63 in each group were detected by real-time fluorescence quantitative PCR, the histomorphology was ob-

给药效率[J].中国医药工业杂志,2016,47(8):976.

（收稿日期:2019-06-21）

2016,39(9)

Gynecol Scand,